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Elephant EEHV Sample Submission Form

Instructions for Elephant Endotheliotropic Herpesvirus (EEHV) Sample Submission

Please submit all samples on plenty of dry ice in a sealed styrofoam container.


For a sick elephant with suspected active EEHV infection, please send at least 1-2 ml of whole blood (EDTA, lavender top tube).  It is best to transfer the blood to a freezer-safe tube (not glass) after thoroughly mixing with EDTA.  We have received broken glass tubes in the past. 

We can test any tissue for EEHV from deceased elephants, but it must be frozen (preferably in liquid nitrogen or dry ice, or store in a –70 C freezer).  Heart, liver and spleen are the best organs to test for EEHV. 

We would like to test placenta from all newborn/stillborn/aborted elephants. Please freeze a 1 inchpiece (preferably in liquid nitrogen or dry ice, or store in a –70 C freezer). 

For EEHV titers, please send at least 2 mls of serum (preferably more).  Freeze immediately and send on dry ice.

Please include all pertinent elephant information (including history) on the attached form. 

The best day to ship (FedEx) would be Wednesday for a Thursday arrival.  Please call Erin or Laura before shipping samples: 

Erin Latimer: 202-633-4252 (W)

        1. (H)

OR      

Laura Richman 301-398-4741 (W)

          301-253-8723 (H)

 FedEx to: 

Erin Latimer/Laura Richman

Smithsonian, National Zoological Park

Department of Pathology

3001 Connecticut Ave. NW

Washington, DC 20008

(202) 633-4252


Request for EEHV testing

Department of Pathology

Smithsonian National Zoo

3001 Connecticut Ave. NW

Washington, DC 20008

(202) 633-4252 

Date ________________________ 

Requestor’s name ______________________________________________________________ 

Institution _____________________________________________________________________ 

Address _______________________________________________________________________ 

      ________________________________________________________________________ 

      ________________________________________________________________________ 

Phone/email ____________________________________________________________________ 
 
 

Animal Name/ISIS # ______________________________________ 

Age ______________________________________________ 

Species ___________________________________________ 

Reason for request  (i.e. current symptoms of possible EEHV, or possible exposure to  

EEHV)  _________________________________________________________________ 

________________________________________________________________________ 

________________________________________________________________________ 

Type of samples being sent __________________________________________________ 

Before sending samples, please call Erin Latimer  202-633-4252 (W)

                                          703-471-2168 (H) 

                              or 

                           Laura Richman 301-398-4741 (W)

                                          301-253-8723 (H)        

Please send samples overnight on dry ice in a sealed cooler.  The best day to send samples is Wednesday, for Thursday delivery.


Consent Form for EEHV Testing

Department of Pathology

Smithsonian National Zoo

3001 Connecticut Ave. NW

Washington, DC 20008

(202) 633-4252 

I give consent for the results of Elephant Endothelial Herpes Virus (EEHV) testing to be used by the Species Survival Plan (SSP) and the National EEHV Laboratory to make recommendations on any elephant related issues (including, but not limited to, recommendations on relocations and breeding). 

The results that could be reviewed and used by the SSP and the National EEHV Lab include Polymerase Chain Reaction (PCR), Enzyme-Linked Immunosorbent Assay (ELISA), and DNA sequencing of the EEHV virus. 

I understand that all results and recommendations will be kept confidential. 
 
 

_____ Yes, I agree to allow the SSP and the National EEHV Lab to use our testing results. 
 

_____ No, I do not consent to the use of our testing results. 
 

__________________________________________  __________
Signature, title                                                                                          Date 
 

___________________________________________________  _________________________
Printed name                                                                                                   Phone number 
 

____________________________________________________  _________________________
Institution                                                                                                         Email address 
 

____________________________________________________
Address 
 

____________________________________________________ 
 
 

____________________________________________________

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