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Canid TAG Necropsy Form

June 1997

American Zoo and Aquarium Association
CANID SSP/TAG NECROPSY PROTOCOL

INSTITUTION/OWNER                                                                                                        

ADDRESS                                                                                                                          

                                                                                                                                       

CANID SPECIES                                   

 

CANID NAME OR ISIS #                                        STUD BOOK #                                 SEX            

BIRTH DATE/AGE                                               WEIGHT                                             

PRODUCED PUPS?                                              HOUSED WITH OPPOSITE SEX?                     

DATE OF DEATH                                                 DATE OF NECROPSY                                  

HISTORY: (briefly summarize clinical signs, circumstances of death):

 

 

 

 

SHIPPING TISSUES: PLEASE OBTAIN PROPER CITES AND EXPORT PERMITS BEFORE SHIPPING TISSUES. 

After 72 hrs in fixative, ship tissues in a leak-proof container in adequate formalin to keep tissues moist. Tissues can be shipped by U.S. Mail or by courier to:

 Dr. Linda Munson
Dept.VM-PMI
1126 Haring Hall
1 Shields Ave
University of California
Davis, CA 95616 
Phone: 530-754-7567
Fax: 530-752-3349
E-MAIL: lmunson@ucdavis.edu

         

GROSS EXAMINATION WORKSHEET

 

PROSECTOR:                                                                                                             

GENERAL CONDITION: (Nutritional condition, physical condition)

Neonates: examine for malformations (cleft palate, deformed limbs etc).

 

 

SKIN: (Including pinna, feet)

 

 

MUSCULOSKELETAL SYSTEM: (Bones, joints, muscles)

 

 

BODY CAVITIES: (Fat stores, abnormal fluids)

Neonates: assess hydration (tissue moistness)

  

HEMOLYMPHATIC: (Spleen, lymph nodes, thymus)

 

 

RESPIRATORY SYSTEM:  (Nasal cavity, larynx, trachea, lungs, regional lymph nodes)

Neonates: determine if breathing occurred (do the lungs float in formalin?)

 

  

CARDIOVASCULAR SYSTEM: (Heart, pericardium, great vessels)


 

DIGESTIVE SYSTEM: (Mouth, teeth, esophagus, stomach, intestines, liver, pancreas, mesenteric lymph nodes).  Neonates: is milk present in stomach?

 

URINARY SYSTEM: (Kidneys, ureters, urinary bladder, urethra)

 

REPRODUCTIVE SYSTEM: (Testis/ovary, uterus, vagina, penis, prepuce, prostate, mammary glands, placenta)

  

ENDOCRINE SYSTEM: (Adrenals, thyroid, parathyroids, pituitary)

 

NERVOUS SYSTEM: (Brain, spinal cord, peripheral nerves)

 

SENSORY ORGANS (Eyes, ears)

 

PRELIMINARY DIAGNOSES:

 

 

 

 

 

 

 

 

LABORATORY STUDIES:(List bacterial and viral cultures submitted and results, if available)

 

 

 

 

FIXED TISSUE CHECK LIST

Preserve the following tissues in 10 % buffered formalin at a ratio of 1 part tissue to 10 parts formalin. Tissues should be no thicker than 1 cm.  INCLUDE SECTIONS OF ALL LESIONS AND SAMPLES OF ALL TISSUES ON THE SSP REQUIRED TISSUE LIST.

 

SSP REQUIRED TISSUES and recommended tissue sampling procedures:

 

__Salivary gland

__Oral/pharyngeal mucosa and tonsil -plus any areas with erosions, ulcerations or proliferative        lesions.

__Tongue - cross section near tip including both mucosal surfaces. 

__Lung - sections from several lobes including a major bronchus

__Trachea

__Thyroid/parathyroids - leave intact.

__Lymph nodes - cervical, mediastinal, bronchial, mesenteric and lumbar. Cut transversely.

__Thymus      

__Heart - longitudinal sections including atrium, ventricle and valves from right and left sides.

__Liver - sections from 3 lobes, including gall bladder

__Spleen - Cross sections including capsule.

__GI Tract - 3 cm long sections of:

Esophagus

Stomach - multiple sections from cardia, fundus (body), and antrum of pylorus

Small intestines - duodenum,  jejunum, ileum

Large intestines - cecum, colon

__Omentum - ~3 cm square

__Pancreas - representative sections from two areas including central ducts

__Adrenal - entire gland with transverse incision.

__Kidney -cortex and medulla from each kidney

__Urinary bladder, ureters, urethra - cross section of bladder and 2 cm sections of ureter and                       urethra.

__Reproductive tract - Entire uterus and ovaries with longitudinal cuts into lumens of  uterine horns.  Both testes (transversely cut) with epididymis.  Entire prostate, transversely cut.     

__Eye - both eyes intact.  Remove extraocular muscles and periorbital tissues.   

__Brain - cut longitudinally along midline. Submit entire brain (retain small part of frontal cortex        frozen if desired) and pituitary gland.

__Spinal cord (if neurologic disease) - sections from cervical, thoracic and lumbar cord.

__Diaphragm and Skeletal muscle - cross section of thigh muscles

__Opened rib or longitudinally sectioned 1/2 femur - marrow must be exposed for proper fixation

__Skin - full thickness of abdominal skin, lip and ear pinna.

__Neonates: umbilical stump - include surrounding tissues.

 

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