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Lion Necropsy Form

LION SSP NECROPSY PROTOCOL for FIV SURVEILLANCE

 

INSTITUTION/OWNER
ADDRESS SEX
LION NAME OR ISIS # STUD BOOK #
BIRTH DATE/AGE WEIGHT
HOUSED WITH OPPOSITE SEX?Y/N PRODUCED CUBS?Y/N
DATE OF DEATH DATE OF NECROPSY

HISTORY: (briefly summarize clinical signs, circumstances of death):

 

 

 

History of (yes/no)?: ABSCESSESDERMATITISCELLULITISSTOMATITIS/GLOSSITISLYMPHADENOPATHYANY RECURRENT INFECTION

 

SHIPPING TISSUES:PLEASE OBTAIN PROPER CITES AND EXPORT PERMITS BEFORE SHIPPING TISSUES.

 

After 72 hrs in fixative, ship tissues in a leak-proof container in adequate formalin to keep tissues moist. Tissues can be shipped by U.S. Mail or by courier to:

 

Dept. Pathology, VTH
College of Veterinary Medicine
University of Tennessee
2407 River Dr.
Knoxville, TN 37996-4500, USA
FAX # 
PHONE # 
E-MAIL:

GROSS EXAMINATION WORKSHEET

PROSECTOR:

 

GENERAL CONDITION: (Nutritional condition, physical condition)
Neonates: examine for malformations (cleft palate, deformed limbs, etc)

 

SKIN: (Including pinna, feet)

 

MUSCULOSKELETAL SYSTEM: (Bones, joints, muscles)

 

BODY CAVITIES: (Fat stores, abnormal fluids)
Neonates: assess hydration (tissue moistness)

 

HEMOLYMPHATIC: (Spleen, lymph nodes, thymus)

 

RESPIRATORY SYSTEM: (Nasal cavity, larynx, trachea, lungs, regional lymph nodes)

 

CARDIOVASCULAR SYSTEM: (Heart, pericardium, great vessels)

 

DIGESTIVE SYSTEM: (Mouth, teeth, esophagus, stomach, intestines, liver, pancreas, mesenteric lymph nodes).
Neonates: is milk present in stomach?

 

URINARY SYSTEM: (Kidneys, ureters, urinary bladder, urethra)

 

REPRODUCTIVE SYSTEM: (Testis/ovary, uterus, vagina, penis, prepuce, prostate, mammary glands, placenta)

 

ENDOCRINE SYSTEM: (Adrenals, thyroid, parathyroids, pituitary)

 

NERVOUS SYSTEM: (Brain, spinal cord, peripheral nerves)

 

SENSORY ORGANS (Eyes, ears)

 

PRELIMINARY DIAGNOSES:

 

 

 

LABORATORY STUDIES:(List bacterial and viral cultures submitted and results, if available)

 

 

 

FIXED TISSUE CHECK LIST

 

Preserve the following tissues in 10 % buffered formalin at a ratio of 1 part tissue to 10 parts formalin. Tissues should be no thicker than 1 cm. INCLUDE SECTIONS OF ALL LESIONS AND SAMPLES OF ALL TISSUES ON THE SSP REQUIRED TISSUE LIST.

 

SSP REQUIRED TISSUES and recommended tissue sampling procedures:

 

__Salivary gland

__Oral/pharyngeal mucosa and tonsil -plus any areas with erosions, ulcerations or proliferative lesions.

__Tongue - cross section near tip including both mucosal surfaces.

__Lung - sections from several lobes including a major bronchus

__Trachea

__Thyroid/parathyroids - leave intact.

__Lymph nodes - cervical, mediastinal, bronchial, mesenteric and lumbar. Cut transversely.

__Thymus

__Heart - longitudinal sections including atrium, ventricle and valves from right and left sides.

__Liver - sections from 3 lobes, including gall bladder

__Spleen - Cross sections including capsule.

__GI Tract - 3 cm long sections of:

__Esophagus

__Stomach - multiple sections from cardia, fundus (body), and antrum of pylorus

__Small intestines - duodenum, jejunum, ileum

__Large intestines - cecum, colon

__Omentum - ~3 cm square

__Pancreas - representative sections from two areas including central ducts

__Adrenal - entire gland with transverse incision.

__Kidney -cortex and medulla from each kidney

__Urinary bladder, ureters, urethra - cross section of bladder and 2 cm sections of ureter and urethra.

__Reproductive tract - Entire uterus and ovaries with longitudinal cuts into lumens of uterine horns. Both testes (transversely cut) with epididymis. Entire prostate, transversely cut.

__Eye - both eyes intact. Remove extraocular muscles and periorbital tissues.

__Brain - cut longitudinally along midline. Submit entire brain (retain small part of frontal cortex frozen if desired) and pituitary gland.

__Spinal cord (if neurologic disease) - sections from cervical, thoracic and lumbar cord.

__Diaphragm and Skeletal muscle - cross section of thigh muscles

__Opened rib or longitudinally sectioned 1/2 femur - marrow must be exposed for proper fixation

__Skin - full thickness of abdominal skin, lip and ear pinna.

__Neonates: umbilical stump - include surrounding tissues.

 

FROZEN TISSUES: The Felid TAG requests that portions of spleen, lymph node, heart and skeletal muscle be stored frozen at your zoo for possible FIV research (-70o C is preferable. Please do not ship).


June 1996 - American Zoo and Aquarium Association

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