American Zoo and Aquarium Association
RED WOLF SSP NECROPSY PROTOCOL
CANID NAME OR ISIS # STUD BOOK # SEX
BIRTH DATE/AGE WEIGHT
PRODUCED PUPS? HOUSED WITH OPPOSITE SEX?
DATE OF DEATH DATE OF NECROPSY
HISTORY: (briefly summarize clinical signs, circumstances of death):
Please have your pathologist perform histopathology on tissues. Then send the gross examination worksheets and pathologist's report to Will Waddell at Point Defiance Zoo.
GROSS EXAMINATION WORKSHEET
GENERAL CONDITION: (Nutritional condition, physical condition)
Neonates: examine for malformations (cleft palate, deformed limbs etc).
SKIN: (Including pinna, feet)
MUSCULOSKELETAL SYSTEM: (Bones, joints, muscles)
BODY CAVITIES: (Fat stores, abnormal fluids)
Neonates: assess hydration (tissue moistness)
HEMOLYMPHATIC: (Spleen, lymph nodes, thymus)
RESPIRATORY SYSTEM: (Nasal cavity, larynx, trachea, lungs, regional lymph nodes)
Neonates: determine if breathing occurred (do the lungs float in formalin?)
CARDIOVASCULAR SYSTEM: (Heart, pericardium, great vessels)
DIGESTIVE SYSTEM: (Mouth, teeth, esophagus, stomach, intestines, liver, pancreas, mesenteric lymph nodes). Neonates: is milk present in stomach?
URINARY SYSTEM: (Kidneys, ureters, urinary bladder, urethra)
REPRODUCTIVE SYSTEM: (Testis/ovary, uterus, vagina, penis, prepuce, prostate, mammary glands, placenta)
ENDOCRINE SYSTEM: (Adrenals, thyroid, parathyroids, pituitary)
NERVOUS SYSTEM: (Brain, spinal cord, peripheral nerves)
SENSORY ORGANS (Eyes, ears)
LABORATORY STUDIES:(List bacterial and viral cultures submitted and results, if available)
FIXED TISSUE CHECK LIST
Preserve the following tissues in 10 % buffered formalin at a ratio of 1 part tissue to 10 parts formalin. Tissues should be no thicker than 1 cm. INCLUDE SECTIONS OF ALL LESIONS AND SAMPLES OF ALL TISSUES ON THE SSP REQUIRED TISSUE LIST.
SSP REQUIRED TISSUES and recommended tissue sampling procedures:
__Oral/pharyngeal mucosa and tonsil -plus any areas with erosions, ulcerations or proliferative lesions.
__Tongue - cross section near tip including both mucosal surfaces.
__Lung - sections from several lobes including a major bronchus
__Thyroid/parathyroids - leave intact.
__Lymph nodes - cervical, mediastinal, bronchial, mesenteric and lumbar. Cut transversely.
__Heart - longitudinal sections including atrium, ventricle and valves from right and left sides.
__Liver - sections from 3 lobes, including gall bladder
__Spleen - Cross sections including capsule.
__GI Tract - 3 cm long sections of:
Stomach - multiple sections from cardia, fundus (body), and antrum of pylorus
Small intestines - duodenum, jejunum, ileum
Large intestines - cecum, colon
__Omentum - ~3 cm square
__Pancreas - representative sections from two areas including central ducts
__Adrenal - entire gland with transverse incision.
__Kidney -cortex and medulla from each kidney
__Urinary bladder, ureters, urethra - cross section of bladder and 2 cm sections of ureter and urethra.
__Reproductive tract - Entire uterus and ovaries with longitudinal cuts into lumens of uterine horns. Both testes (transversely cut) with epididymis. Entire prostate, transversely cut.
__Eye - both eyes intact. Remove extraocular muscles and periorbital tissues.
__Brain - cut longitudinally along midline. Submit entire brain (retain small part of frontal cortex frozen if desired) and pituitary gland.
__Spinal cord (if neurologic disease) - sections from cervical, thoracic and lumbar cord.
__Diaphragm and Skeletal muscle - cross section of thigh muscles
__Opened rib or longitudinally sectioned 1/2 femur - marrow must be exposed for proper fixation
__Skin - full thickness of abdominal skin, lip and ear pinna.
FROZEN TISSUE: Store in plastic bags at -70 or -20 C for toxicology: Liver, brain, kidney and (if possible) antemortem serum and plasma frozen. If you suspect an infectious disease, also freeze samples of small intestines, lung, spleen, and lymph nodes.
NEONATAL NECROPSY PROTOCOL
Please follow the adult protocol in addition to the following:
1. Check umbilical stump and surrounding tissues
2. Check for malformations (cleft palate, deformed limbs, heart defects)
3. Assess hydration (tissue moistness) and evidence of nursing (milk in stomach).
4. Determine if breathing occurred (do the lungs float in formalin?)
5. Check foot pads for erosions and ulcers
ADDITIONAL TISSUES FOR HISTOPATHOLOGY FROM NEONATES:
All tissues from the adult necropsy check-list.
Umbilicus (including external and internal vessels and surrounding skin.
Foot pads from all feet.
Extra sections of lung.