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vet_nutrition_tissue_blood_collect_protocol

 

 

VETERINARY/NUTRITION BLOOD AND TISSUE COLLECTION PROTOCOL FOR RHINOCEROS

January 2004

 

 

 

 

 

Michele Miller, DVM, PhD

Veterinary Advisor, Black Rhinoceros SSP

Disney’s Animal Kingdom

P.O. Box 10,000

Lake Buena Vista, FL  32830-1000

(407) 939-7316

FAX:  (407) 938-1909

Email:  Michele.Miller@disney.com

 

R. Eric Miller, DVM, Dipl. ACZM

Veterinary Advisor, Rhinoceros TAG

St. Louis Zoo

1 Government Drive

St. Louis, MO  63110-1396

(314) 781-0900 ex. 483

FAX:  (314) 768-5454

Email:  remiller@stlzoo.org

 

 


 

TABLE OF CONTENTS

 

Introduction:  An overview of how to use this protocol                                   3

 

Submission form (to copy and accompany all shipments)                            4-5                  

 

Summary of tests and samples requested                                                         6-8

            Blood collection samples                                                                        6

            Skin biopsies                                                                                                7

            Sperm bank samples                                                                            7

            Necropsy tissues                                                                                  7-8

            Individuals interested in participating in rhino necropsies                    8

 

Checklist of samples to collect                                                              9-13

            Routine blood sampling                                                             9

            Necropsy tissue checklist                                                                       10-11

            Diagnostic testing for specific disease cases                                        11-13

 

Summary of research project information                                                       14-16

           

Veterinary and scientific advisors-rhinoceros SSPs and TAGs               17

 

Directory of addresses of researchers and scientific advisors                        18-23

 


 

INTRODUCTION

HOW TO USE THIS MANUAL

 

Thank you for your efforts to collect samples for the numerous research projects regarding the health and nutrition of rhinoceroses.  To date, your cooperative efforts have supplied a variety of research projects with material for their studies.  Numerous scientific publications and presentations have resulted.  Most importantly, these findings are building a firmer base for our understanding of rhinoceros health and nutrition.

 

To submit samples, just go through the following steps:

 

  1. Collect the appropriate sample as outlined in the “Summary of Tests and Samples on pages 6-8.
  2. Make copies of the “Submission Form” (pages 4-5) for each animal and collection date.  Once it is completed, it can be copied and mailed to each researcher.
  3. Store or ship the samples as outlined in the “Summary of Tests and Samples” on pages 6-8.  Addresses appropriate for express shipments are listed in the Directory of Researchers and Veterinary Advisors (pages 18-23).
  4. If collecting samples for a suspected specific disease, check the list of recommended diagnostic and research samples on pages 11-13.
  5. If possible, contact each researcher prior to shipment of samples.  Names and phone numbers, email and FAX numbers are located in the Directory of Researchers and Scientific Advisors (pages 18-23).  NOTE:  some of the tests listed require a lab fee; please check the listed information carefully.  An asterik (*) indicates a fee-based test.  Call the contact individual with any specific questions.
  6. If you have specific questions, consult with the individual included in the description of each project.  If you have additional questions, please contact:

           

Michele Miller

Veterinary advisor, Black rhinoceros SSP

Phone:  407-939-7316

FAX:  407-938-1909

Email:  Michele.Miller@disney.com

 

OR

 

R. Eric Miller

Veterinary Advisor, Rhinoceros TAG

Phone:  314-781-0900 ext. 483

FAX:  314-768-5454

Email:  remiller@stlzoo.org

 


 

RHINOCEROS SAMPLE SUBMISSION FORM

(MAKE EXTRA COPIES AS NEEDED)

 

Please fill out one form for sample collection date.  Please make copies and send one to each researcher that receives samples.

 

SUBMITTER:  __________________________________

 

                          __________________________________

 

                          __________________________________

 

                          __________________________________

 

PHONE/FAX:   ___________________________________________________

 

EMAIL:            ___________________________________________________

 

SPECIES:         ___________________________________________________

(If possible, please indicate subspecies; e.g., eastern or southern black rhinoceros)

 

SEX:                 ___________          ANIMAL NAME:  ___________________

 

IDENTIFICATION NUMBER:  _____________  (Studbook number is preferred)

 

DATE OF BIRTH (MON/DAY/YR):  ___________________

(If unknown, give approx. age)    

 

DATE SAMPLE COLLECTED (MON/DAY/YR):  __________________

 

SAMPLE COLLECTION INFORMATION:  (check all that apply)

__serum  __hep. plasma __ EDTA plasma 

__EDTA whole blood __hep whole blood __ other: specify _________________

 

Volume _____________     Tissue(s) ___________________________________

 

Processed within:  __ <2 hrs  __ 2-12 hrs  __ >12 hrs

Sample storage:  __ frozen immediately  __ refrigerated  __ room temp.

__ other: specify ___________________________________________________

 

Sample collected:  __ live animal  __ post-mortem

 

 

 

 

 

REASON SAMPLE COLLECTED (ROUTINE EXAM, ILL, ETC):

 

 

 

 

 

 

 

OTHER RELEVANT INFORMATION:

 

 

 

 

 

 

 

If you have questions, please call Michele Miller @ 407-939-7316 or Eric Miller @ 314-781-0900 ext. 483.

 


 

SUMMARY OF TESTS AND SAMPLES

 

Codes:

ON = overnight express

DI   = dry ice

WI  = wet ice

 

BLOOD COLLECTION SAMPLES

 

Researcher/Test                  Sample                  Time of Shipment                  How to Ship

 

Dr. Eric Miller/            10+ ml each            Freeze (-70 C)            ON, DI

Serum bank            hep plasma &            Ship at zoo’s convenience

            serum

 

Dr. Dierenfeld/            5 ml EDTA           Freeze (-70 C)                  ON, DI Nutrition            plasma &                  Ship at zoo’s convenience

            3 ml hep plasma

 

Dr. Ball/            1-2 ml serum            Freeze (-70 C)                  ON, WI      

Phospholipid syndrome                Ship at zoo’s convenience    

 

 

            Dr. Michele Miller            10 ml serum            Freeze (-70 C)                   ON, DI

            Infectious/immune dz   


 

SKIN BIOPSIES

 

Researcher/Test                  Sample                  Time of Shipment                  How to Ship

 

Dr. Munson/                  Skin biopsies of                   Ship at zoo’s convenience                  Regular US

Ulcerative syndrome                  affected areas in                                          mail (fixed

                  formalin,                                         tissues)

                  glutaraldehyde,                                                

                  and frozen                                     ON, DI

(frozen tissue)

 

                  1-2 ml hep plasma                  Ship at zoo’s convenience                  ON, DI

 

SPERM BANK SAMPLES

 

Researcher/Test                  Sample                  Time of Shipment                  How to Ship

 

Dr. Terri Roth/                  Both testicles                  Ship day of collection                  ON, WI

Sperm Rescue                with epididymus     

                                      and vas deferens

 

NECROPSY TISSUES

 

Researcher/Test                  Sample                  Time of Shipment                  How to Ship

 

Dr. Mary Duncan/                Full tissue set in      Ship at zoo’s convenience         Regular US

Pathology                              formalin plus                                                                 mail

*Black rhino only                  sections of main

                  organs in

                  glutaraldehyde

 

Dr. Mike Kinsel                  Full tissue set in                  Ship at zoo’s convenience                  Regular US

Pathology                  formalin                                          mail

*White rhino only                  50-100 g of                  Freeze (-70C)                  ON, DI

                  liver, spleen,                   Ship at zoo’s convenience

                  kidney, heart,

                  lung, brain

 

Dr. Trupkiewicz/                  Full tissue set in                   Ship at zoo’s convenience                  Regular US

Pathology                  formalin                                          mail

*Indian rhino only

 

Dr. Linda Lowenstein Full tissue set in                  Ship at zoo’s convenience                  Regular US

Pathology                  formalin

*Sumatran rhino only

 

Dr. Atkinson                  Intact pelvic limb                   Contact investigator

*Indian rhino only                  distal to the tarsus

 

Dr. Dierenfeld/                  10 grams each:                  Freeze (-70 C)                  ON, DI

Nutrition                  liver, heart,                  Ship at zoo’s convenience

                  Skeletal muscle,                                                                                                 adipose

 

Dr. Eric Miller/                  200 grams each:                  Freeze (-70 C)                  ON, DI

Tissue bank                  liver, kidney,                   Ship at zoo’s convenience

                  spleen, gut

                  contents

 

 

Dr. Eric Miller/                  ½ brain in formalin                  Ship at zoo’s convenience                  Regular US

Encephalomalacia                                                             mail

                  ½ brain frozen (-70 C)                                ON, DI

 

 

 

INDIVIDUALS INTERESTED IN PARTICIPATING IN RHINO NECROPSIES

 

Dr. Don Paglia                  See contact information

Would appreciate opportunity to participate in necropsy of any rhinoceros, regardless of species, geographic location, or time.

 


 

CHECKLIST OF SAMPLES TO COLLECT

 

ROUTINE BLOOD SAMPLING (INCLUDING SICK RHINOS)

Sample                        Volume            Investigator                     Shipping            Project

 

                                   

Heparinized                  10+ ml             Dr. Eric Miller               ON, DI                        Serum bank

plasma                          (2-3 x 10 ml hep blood-separate plasma and freeze – 70 C)

 

                                    3 ml                  Dr. Dierenfeld                     ON, DI                        Nutrition

                                    (7 ml hep blood-separate plasma and freeze at –70 C)

 

Serum                          10 ml                Dr. Michele Miller            ON, DI                        Infect dis

                                    (3 x 10 ml clot tube-separate serum and freeze at –70C)

 

                                    10+ ml             Dr. Eric Miller               ON, DI                        Serum bank

                                    (3-4 x 10 ml clot tube-separate serum and freeze at –70C)

                       

                                    1-2 ml              Dr. Ray Ball                  ON, WI            Phospholipid

                       

EDTA  plasma              5 ml                  Dr. Dierenfeld                     ON, DI                        Nutrition

                                    (1-2 x 10 ml EDTA blood-separate plasma and freeze at –70C)

 

Totals for research projects: 

            3-4 x 10 ml heparin tubes –             separate plasma and freeze

            7-9 x 10 ml clot tubes –             separate serum and freeze

            1-2 x 10 ml EDTA tubes –             separate plasma and freeze

           

 

 


 

 NECROPSY TISSUE CHECKLIST

All Rhino Species

Tissue             Amount            Investigator                     Shipping            Project

 

Liver-frozen

                        200 grams   Dr. Eric Miller               ON, DI                        Tissue bank

           

                        5-10 grams   Dr. Dierenfeld              ON, DI                        Nutrition

 

Spleen-frozen  

                        200 grams   Dr. Eric Miller               ON, DI                        Tissue bank

                       

Kidney-frozen

                        200 grams   Dr. Eric Miller               ON, DI                        Tissue bank

 

 

Gut contents-frozen  

                        200 grams   Dr. Eric Miller               ON, DI                        Tissue bank

 

Brain-frozen

                        200 grams   Dr. Eric Miller               ON, DI                        Tissue bank

 

Heart-frozen

                        5-10 grams   Dr. Dierenfeld                     ON, DI                        Nutrition

 

Skeletal muscle-frozen

                        5-10 grams   Dr. Dierenfeld                     ON, DI                        Nutrition

 

Adipose tissue-frozen

                        5-10 grams   Dr. Dierenfeld                     ON, DI                        Nutrition

 

Testicles-fresh/cooled     

                        Both testes    Dr. Terri Roth                     ON, WI                   Sperm Bank


 

 

Black Rhinoceros Only

Tissue                                                       Investigator         Shipping            Project

 

Complete set of formalinized tissues              Dr. Duncan US Mail            Pathology

 or slide set

 

1 mm squares of major tissues             Dr. Duncan US Mail            Pathology

in glutaraldehyde

 

Greater Asian One-Horned Rhinoceros Only

Tissue                                                       Investigator         Shipping            Project

 

Complete set of formalinized tissues              Dr. Trupkiewicz   US Mail            Pathology

 

Intact pelvic limb from tarsus distally  Dr. Atkinson            Call for information

           

White Rhinoceros Only

Tissue                                                       Investigator         Shipping            Project

 

Complete set of formalinized tissues              Dr. Kinsel   US Mail            Pathology

 

Liver, kidney, spleen, heart, lung, brain-            Dr. Kinsel   ON, DI                        Pathology

50-100 grams of each, frozen at –70C

 

Sumatran Rhinoceros Only

Tissue                                                       Investigator         Shipping            Project

 

Complete set of formalinized tissues              Dr. Lowenstein US Mail            Pathology

 

 

Diagnostic Testing/Necropsy of Suspected Specific Disease Cases in All Rhino Species (* indicates fee-based test – paid by submitting institution)

Tissue                                                             Investigator            Shipping       

 

Skin biopsy/mucocutaneous ulcers ONLY

Biopsies in formalin,                                   Dr. Linda Munson            US mail for all except frozen

glutaraldehyde,                                                                         tissue (send ON, DI)

frozen (-70C)

-         biopsies should include lesion’s edge

 

2 ml hep plasma                          Dr. Linda Munson            ON, DI


 

Idiopathic hemorrhagic vasculopathy syndrome (IHVS)

Send copy of results to Dr. Michele Miller

 

1 ml serum (frozen)                        Dr. Angulo                        ON, WI                                                TVMDL                       

                                                            Phone:            888-646-5623        

                                                            Request test for cold agglutinins*

 

Nasal/respiratory swab                        Submit for Streptococcus culture at local lab*     

 

5-10 ml serum                                      Dr. Michele Miller            ON, DI

                                                            Serum bank for future investigations

 

Leptospirosis cases/hemolytic anemia cases

Liver – 50 grams frozen                             Dr. Bolin    ON, DI                        Leptospirosis

                                                                                                                        fluorescent

                                                                                                                        antibody*

 

2 ml serum                                                        Dr. Bolin    ON, DI                   Lepto titer*

 

20 ml urine                                                       Dr. Harkin  ON, WI                   Lepto PCR*

Kidney - frozen

 

Encephalomalacia

Brain – ½ in formalin                                                Dr. Eric Miller   US Mail

            100-200 grams frozen                                                     ON, DI

 

Feed samples – frozen  at –70C                        Dr. Eric Miller     ON, DI

 

Liver – 100 grams frozen at –70C               Dr. Eric Miller  ON, DI

 

Suspected iron-storage disease

Due to changes in labs, testing for suspected imbalances in iron metabolism is only available through KSU as a diagnostic test (fee-based).  *Tests available: serum iron, TIBC, ferritin, transferrin saturation, haptoglobin.

 

2 ml serum                                            Dr. Sue Chavey ON, WI

                                                            KSU Vet. Med. Center, Mosier Hall

                                                            Comparative Hematology

                                                            1800 Denison Avenue

                                                            Manhattan, KS  66506-5605

                                                            Phone:  785-532-4424


 

                                               

Suspected vitamin D toxicosis

*Diagnostic testing is fee-based.  Send copy of results to Dr. Michele Miller.

 

1 ml serum (frozen)                                    MSU-VDL                ON, WI                              

                                                            Phone:  517-353-1683

 

*Indicates charge for testing – contact investigator for current information


 

SUMMARY OF RESEARCH PROJECT INFORMATION

 

Please review shipping information carefully.  Unless otherwise noted, all shipping is paid by submitter.  Some tests may require additional lab fees – contact investigator PRIOR to shipping.

 

Serum and Tissue Bank – Dr. Eric Miller

 

Dr. Miller has maintained a serum and tissue bank for the Rhinoceros TAG.  These materials are vital to maintaining a central sera bank for all rhinoceros species. 

 

Shipping information for samples:

Ship to address listed in investigator directory (Dr. Eric Miller).  The samples can be frozen locally and shipped at a later date, frozen, overnight on dry ice.

 

 

Nutritional Analyses – Dr. Ellen Dierenfeld

 

Dr. Dierenfeld continues to measure vitamin A&E, fatty acid and mineral levels in plasma and tissues in all rhinoceros species.  This provides baseline information for these species, including samples from free-ranging animals.

 

Shipping information for samples:

            For all species, it would be useful to have detailed information on diets when samples are taken; include accurate estimates of the amounts of hays (grass, mixed, legume), browse (including species), pellets or other concentrates, produce, and any supplements consumed per animal per day. 

Ship to address listed in investigator directory (Dr. Ellen Dierenfeld).  For blood samples, please separate plasma and freeze at –70C; tissue samples should also be frozen.  All samples should be shipped overnight on dry ice. *Lab tests may have associated fee – contact investigator for current information.

 

Investigation of Leptospirosis Cases – Dr. Carole Bolin and Dr. Ken Harkin

 

For suspected cases of leptospirosis, tissues and serum should be sent to Dr. Bolin and urine or kidney sent to Dr. Harkin for additional diagnostics.  The fluorescent antibody test performed on liver has been the most sensitive test for leptospiral infection and has identified cases that were negative by microagglutination titer.  Any cases of hemolytic anemia should also be tested for leptospiral infection.

 

Shipping information:

Ship to address listed in investigator directory (Dr. Carole Bolin and Dr. Ken Harkin).  Please include vaccination history with leptospiral bacterins in submission information.  Samples (liver, kidney, and serum) can be frozen at –70C and shipped overnight on dry ice.  Urine can be shipped cool or frozen overnight on wet ice.  *Lab tests are performed for diagnostic purposes and require submitter to pay lab fees – contact investigators for current information.

 

Investigation of Ulcerative Dermatitis Cases – Dr. Linda Munson

 

Dr. Munson is reviewing biopsies from all cases of mucocutaneous syndrome.  Duplicate slides or tissue samples should be submitted to her along with your regular pathologist.

 

Shipping information:

            Ship to address listed in investigator directory (Dr. Linda Munson).  Tissues placed in formalin and glutaraldehyde can be sent by US mail.  Duplicate tissue samples and heparinized plasma should be frozen at –70C and shipped overnight on dry ice.

 

Investigation of Encephalomalacia Cases – Dr. Eric Miller

 

Although encephalomalacia has occurred primarily in young animals, it should be considered in all deaths of unknown cause.  Symptoms can vary from hyperexcitability to hyperthermia, to a nearly comatose state.

 

Shipping information:

            Ship to address listed in investigator directory (Dr. Eric Miller).  Frozen samples should be shipped overnight on dry ice.  Formalin tissues can be sent by US mail.

 

Serosurvey of Infectious Diseases – Dr. Michele Miller

 

Although serologic surveys of small numbers of captive rhinoceros have been performed, a comprehensive survey of multiple infectious diseases and immune parameters has not been completed.  The goal of this project is provide current information on exposure to infectious diseases and immune parameters in the captive population.

 

Shipping information:

            Ship to address listed in investigator directory (Dr. Michele Miller).  Frozen samples should be shipped overnight on dry ice.

 

Evaluation of Antiphospholipid Syndrome – Dr. Ray Ball

 

The antiphospholipid syndrome (APS) is defined as the occurrence of thrombosis, fetal losses, and moderate thrombocytopenia in the presence of phospholipid antibodies.  Samples are requested from black rhinos with any of the syndromes affecting this species in captivity to investigate a possible association with APS.  See 2002 SSP report for more details.

 

Shipping information:

            Ship to address listed in investigator directory (Dr. Ray Ball).  Frozen samples should be shipped overnight on dry ice or ice packs.  Dr. Ball will pay shipping fees if contacted prior to shipping.

 

Evaluation of Foot Lesions and Pathology in Greater Asian One-Horned Rhinoceros – Dr. Mark Atkinson

 

Dr. Atkinson requests that one intact distal extremity of the pelvic limb (distal to the tarsus) of any Asian one-horned rhinoceros be shipped to him for further studies on foot lesions and pathology affecting this species.

 

Shipping information:

Please contact Dr. Atkinson for information on preserving and shipping the requested material.  See contact information in the investigator directory.

 

Sperm Rescue and Banking – Dr. Terri Roth

 

Dr. Roth has developed successful methods for cryopreserving epididymal sperm samples and is now actively establishing a rhino sperm bank.  Genetically valuable (under-represented) males of all species are especially important.

 

Shipping information:

            Ship to address listed in investigator directory (Dr. Terri Roth).  Remove testes from animal as soon as possible following death.  Place in zip-lock baggies with saline-moistened gauze.  Surround plastic bag with paper towels for insulation then place in styrofoam cooler on cold packs and ship overnight.

 

 

VETERINARY AND SCIENTIFIC ADVISORS

RHINOCEROS SSPs AND TAGs

 

Rhinoceros Taxon Advisory Group (TAG)

            TAG Chair:  Dr. Evan Blumer

            Research Advisor:  Dr. Evan Blumer

            Veterinary Advisor:  Dr. Eric Miller

            Nutritional Advisor:  Dr. Ellen Dierenfeld

 

Black Rhinoceros SSP

            SSP Coordinator:  Dr. Don Farst

            Veterinary Advisor:  Dr. Michele Miller

            Pathology Advisor:  Dr. Mary Duncan

            Nutritional Advisor:  Dr. Ellen Dierenfeld

 

White Rhinoceros SSP

            SSP Coordinator:  Michael Fouraker

            Veterinary Advisor:  Dr. Michael Briggs

            Pathology Advisor:  Dr. Michael Kinsel

            Nutritional Advisor:  Dr. Michael Schlegel

 

Greater Asian One-Horned Rhinoceros SSP

            Species Coordinator:  Michael Dee

            Veterinary Advisor:  Dr. Mark Atkinson

            Pathology Advisor:  Dr. John Trupkiewicz

            Nutritional Advisor:  Kerrin Grant

 

Sumatran Rhinoceros

            Species Coordinator:  James Doherty and James Dolan

            Veterinary Advisor:  Dr. Mark Campbell

            Pathology Advisor:  Dr. Linda Lowenstein

            Nutritional Advisor:  Dr. Ellen Dierenfeld

 

Other advisors:

            Dr. Robin Radcliffe

            Dr. Steve Osofsky


 

ALPHABETICAL DIRECTORY OF ADDRESSES OF RESEARCHERS AND SCIENTIFIC ADVISORS

 

Dr. Mark Atkinson

The Wilds

14000 International Road

Cumberland, OH  43732

Phone:  740-638-2073

FAX:  740-638-2287

Email:  matkinson@thewilds.org

 

Dr. Ray Ball

Busch Gardens Tampa Bay

3605 Bougainvillea Drive

Tampa, FL  33674

Phone:  813-987-5562

FAX:  813-987-5548

Email:  Dr.Ray.Ball@BuschGardens.com

 

Dr. Evan Blumer

The Wilds

14000 International Road

Cumberland, OH  43732

Phone:  740-638-2284

FAX:  740-638-2287

Email:  eblumer@thewilds.org

 

Dr. Carole Bolin

Diagnostic Center for Population and Animal Health

A3 Veterinary Medical Center

College of Veterinary Medicine

Michigan State University

East Lansing, MI  48824

Phone:  517-355-8533

FAX:  517-432-9813

Email:  bolinc@dcpah.msu.edu

 

Dr. Michael Briggs

Brookfield Zoo

3300 Golf Road

Brookfield, IL  60513-1060

Phone:  708-485-0263 ext. 501

FAX:  708-485-0269

Email:  mbbriggs@ix.netcom.com

 

 

Dr. Mark Campbell

Cincinnati Zoological and Botanical Gardens

3400 Vine Street

Cincinnati, OH  45220

Phone:  513-559-7751

FAX:  513-559-7773

Email:  mark.campbell@cincinnatizoo.org

 

Michael Dee

Los Angeles Zoo

5333 Zoo Drive

Los Angeles, CA  90027

Phone:  323-644-4254

Email:  mdee@zoo.ci.la.ca.us

 

Dr. Ellen Dierenfeld

St. Louis Zoological Park

Veterinary Hospital

1 Government Drive, Forest Park

St. Louis, MO  63110

Phone:  314-781-0900 ext. 205

Email:  dierenfeld@stlzoo.org

 

 

James Doherty

Bronx Zoo

2300 Southern Blvd.

Bronx, NY  10460

Phone:  718-220-7111

Email:  jdoherty@wcs.org

 

James Dolan

San Diego Zoo

P.O. Box 120551

San Diego, CA  92112

Phone:  619-557-3909

 

Dr. Mary Duncan

St. Louis Zoological Park

Veterinary Hospital

1 Government Drive, Forest Park

St. Louis, MO  63110

Phone:  314-781-0900 ext. 234

Email:  duncan@stlzoo.org

 

Dr. Don Farst

Gladys Porter Zoo

500 Ringgold Street

Brownsville, TX  78520

Email:  dfarst@gpz.org

 

Dr. Tom Foose

Program Director

International Rhino Foundation (IRF)

20 Pen Mar Street

Waynesboro, PA  17268

Phone:  717-765-9373

FAX:   717-765-9375

Email:  irftom@aol.com

 

Michael Fouraker

Fort Worth Zoo

1989 Colonial Parkway

Fort Worth, TX  76110

Phone:  817-759-7590

Email:  mfouraker@fortworthzoo.org

 

Kerrin Grant

Wildlife Rehabilitation Center

2675 Norkenzie Road

Eugene, OR  97408

Phone:  541-685-9397

Email:  selasphorus@msn.com

 

Dr. Ken Harkin

Email:  Harkin@vet.k-state.edu

Note contact info for lab below:

College of Veterinary Medicine

Kansas State University

Veterinary Diagnostic Lab

1800 Denison Ave.

Manhattan, KS  66506

Phone:  785-532-5650

 

Dr. Michael Kinsel

University of Illinois

Zoological Pathology Program

LUMC, Bldg 101, Rm 0745

2160 S. First Ave.

Maywood, IL  60153

Ship tissues to:

Brookfield Zoo

Attn:  Dr. Kinsel

3300 Golf Road

Brookfield, IL  60513-1060

Phone:  708-216-1185

FAX:  708-216-5934

Email:  mkinsel@lumc.edu

 

Dr. Linda Lowenstine

Dept. of Pathology

School of Veterinary Medicine

University of California-Davis

Davis, CA  95616

Phone:  530-752-1182

FAX:  530-752-3349

Email:  ljlowenstine@ucdavis.edu

 

Dr. Eric Miller

St. Louis Zoo

1 Government Drive

St. Louis, MO  63110-1396

Phone:  314-781-0900 ext. 483

FAX:  314-768-5454

Email:  remiller@stlzoo.org

 

Dr. Michele Miller

Disney’s Animal Kingdom

Dept. of Veterinary Services

1200 N. Savannah Circle East

Bay Lake, FL  32830-1000

Phone:  407-939-7316

FAX:  407-938-1909

Email:  Michele.Miller@disney.com

 

Dr. Linda Munson

Department of Pathology

School of Veterinary Medicine

University of California-Davis

Davis, CA  95616

Phone:  530-754-7567

FAX:  530-752-3349

Email:  lmunson@ucdavis.edu

 

Dr. Steve Osofsky

WCS Field Program

11697 Fox Glen Drive

Oakton, VA  22124

Phone:  703-716-1029

Email:  sosofsky@wcs.org

 

Dr. Don Paglia

Phone:  818-788-0854

Email:  dpaglia@mcn.org

 

Dr. Robin Radcliffe

Fossil Rim Wildlife Center

P.O. Box 2189

2155 County Road 2008

Glen Rose, TX  76043-6117

Phone:  254-897-2960 ext. 313

FAX:  254-897-3785

Email:  RobinR@fossilrim.org

 

Dr. Terri Roth

Cincinnati Zoo & Botanical Garden

Center for Conservation and Research of Endangered Wildlife (CREW)

3400 Vine Street

Cincinnati, OH  45220

Phone:  513-569-8220

FAX:  513-569-8213

Email:  terri.roth@cincinnatizoo.org

 

Dr. Michael Schlegel

Escondido, CA  92026

Phone:  760-735-8014

Email:  schlegem@yahoo.com

 

Dr. John Trupkiewicz

Philadelphia Zoo

3400 W. Girard Ave.

Philadelphia, PA 19104

Phone:  215-243-5324

FAX:    215-243-0219

Email:  Trupkiewicz.John@PhillyZoo.org

 

 

1/30/04 mm

Location

581705 White Oak Road
Yulee, FL 32097 USA

Contact Us

Local: (904) 225-3275
Fax: (904) 225-3289
Email:Admin@AAZV.org