Handling of Small Cat Reproductive Tissue for Gamete Rescue
Center for Conservation and Research of Endangered Wildlife
Cincinnati Zoo and Botanical Garden
- Oocytes remain viable in the ovary for ~24 h including time of shipment.ÊTissue from females found >12 h after death should be sent directly to Dr. Karen Terio.
- Spermatozoa will remain viable for slightly longer depending on temperature.ÊTissues from males can be used if found within ~18 h, if ambient temperature is 30¡ C.
- Gonads from Pallas cats will only be useful if recovered November thru April due to their reproductive seasonality.
- As soon as possible after death, remove the ovaries or testes.ÊFor testes, please include most of the spermatic cord.
- Place the gonads in a clean, preferably sterile, container (specimen cup, ziplock bag, test tube, etc.) and cover with enough sterile saline to keep the tissue moist in transit.
- Place the samples in a small styrofoam cooler along with 1-2 ice packs.ÊWrap the ice packs in several layers of paper towel so the samples do not get cooler than ~5¡ C.ÊExcess space can be filled with styrofoam pellets or bubble wrap, but do not leave too much space between the samples and the ice packs.
Jason Herrick (Post-Doc, email@example.com, 513-569-8208)
Bill Swanson (Head of Animal Conservation, firstname.lastname@example.org, 513-569-8213)
Helen Bateman (Research Associate, email@example.com, 513-569-8210), or Jennifer Bond (Laboratory Technician, firstname.lastname@example.org, 513-569-8209)
**If the samples are from pre-pubertal animals, we may request that they be sent to other researchers.**
Ship the samples Priority Overnight via FedEx.