American Zoo and Aquarium Association
CANID SSP/TAG NECROPSY PROTOCOL
CANID NAME OR ISIS # STUD BOOK # SEX
BIRTH DATE/AGE WEIGHT
PRODUCED PUPS? HOUSED WITH OPPOSITE SEX?
DATE OF DEATH DATE OF NECROPSY
HISTORY: (briefly summarize clinical signs, circumstances of death):
SHIPPING TISSUES: PLEASE OBTAIN PROPER CITES AND EXPORT PERMITS BEFORE SHIPPING TISSUES.
After 72 hrs in fixative, ship tissues in a leak-proof container in adequate formalin to keep tissues moist. Tissues can be shipped by U.S. Mail or by courier to:
Dr. Linda Munson
1126 Haring Hall
1 Shields Ave
University of California
Davis, CA 95616
GROSS EXAMINATION WORKSHEET
GENERAL CONDITION: (Nutritional condition, physical condition)
Neonates: examine for malformations (cleft palate, deformed limbs etc).
SKIN: (Including pinna, feet)
MUSCULOSKELETAL SYSTEM: (Bones, joints, muscles)
BODY CAVITIES: (Fat stores, abnormal fluids)
Neonates: assess hydration (tissue moistness)
HEMOLYMPHATIC: (Spleen, lymph nodes, thymus)
RESPIRATORY SYSTEM: (Nasal cavity, larynx, trachea, lungs, regional lymph nodes)
Neonates: determine if breathing occurred (do the lungs float in formalin?)
CARDIOVASCULAR SYSTEM: (Heart, pericardium, great vessels)
DIGESTIVE SYSTEM: (Mouth, teeth, esophagus, stomach, intestines, liver, pancreas, mesenteric lymph nodes). Neonates: is milk present in stomach?
URINARY SYSTEM: (Kidneys, ureters, urinary bladder, urethra)
REPRODUCTIVE SYSTEM: (Testis/ovary, uterus, vagina, penis, prepuce, prostate, mammary glands, placenta)
ENDOCRINE SYSTEM: (Adrenals, thyroid, parathyroids, pituitary)
NERVOUS SYSTEM: (Brain, spinal cord, peripheral nerves)
SENSORY ORGANS (Eyes, ears)
LABORATORY STUDIES:(List bacterial and viral cultures submitted and results, if available)
FIXED TISSUE CHECK LIST
Preserve the following tissues in 10 % buffered formalin at a ratio of 1 part tissue to 10 parts formalin. Tissues should be no thicker than 1 cm. INCLUDE SECTIONS OF ALL LESIONS AND SAMPLES OF ALL TISSUES ON THE SSP REQUIRED TISSUE LIST.
SSP REQUIRED TISSUES and recommended tissue sampling procedures:
__Oral/pharyngeal mucosa and tonsil -plus any areas with erosions, ulcerations or proliferative lesions.
__Tongue - cross section near tip including both mucosal surfaces.
__Lung - sections from several lobes including a major bronchus
__Thyroid/parathyroids - leave intact.
__Lymph nodes - cervical, mediastinal, bronchial, mesenteric and lumbar. Cut transversely.
__Heart - longitudinal sections including atrium, ventricle and valves from right and left sides.
__Liver - sections from 3 lobes, including gall bladder
__Spleen - Cross sections including capsule.
__GI Tract - 3 cm long sections of:
Stomach - multiple sections from cardia, fundus (body), and antrum of pylorus
Small intestines - duodenum, jejunum, ileum
Large intestines - cecum, colon
__Omentum - ~3 cm square
__Pancreas - representative sections from two areas including central ducts
__Adrenal - entire gland with transverse incision.
__Kidney -cortex and medulla from each kidney
__Urinary bladder, ureters, urethra - cross section of bladder and 2 cm sections of ureter and urethra.
__Reproductive tract - Entire uterus and ovaries with longitudinal cuts into lumens of uterine horns. Both testes (transversely cut) with epididymis. Entire prostate, transversely cut.
__Eye - both eyes intact. Remove extraocular muscles and periorbital tissues.
__Brain - cut longitudinally along midline. Submit entire brain (retain small part of frontal cortex frozen if desired) and pituitary gland.
__Spinal cord (if neurologic disease) - sections from cervical, thoracic and lumbar cord.
__Diaphragm and Skeletal muscle - cross section of thigh muscles
__Opened rib or longitudinally sectioned 1/2 femur - marrow must be exposed for proper fixation
__Skin - full thickness of abdominal skin, lip and ear pinna.
__Neonates: umbilical stump - include surrounding tissues.